ICCBM-16 Programme

Prague, Hotel Pyramida, July 2-7, 2016

Programme overview - sessions (pdf) Programme overview - speakers (pdf)
Programme detailed (pdf)

Saturday, July 2

10:00-19.00   Exhibition installation
15:00-22.00   Registration
20:00-22.00   Welcome reception

Sunday, July 3

8:30 Opening ceremony    

chair: Claude Sauter



Osamu Nureki
Graduate School of Science, University of Tokio, Japan

High-resolution X-ray Crystallography of Membrane Proteins and Molecular Mechanisms of Membrane Transporters



S1 - Membrane Protein Crystallization I

chair: Martin Caffrey

9:50 Eric Xu 
Van Andel Institute in Michigan
An X-Ray Laser Structure of Rhodopsin-Arrestin Complex htm pdf
10:20 Byron Carpenter
Cambridge, UK
Engineering Heterotrimeric G Proteins to Facilitate Crystallisation of GPCRs in their Active Conformation htm pdf
10:50 Martin Caffrey
Trinity College, Dublin, Ireland
New and Improved Features of the Lipid Cubic Phase (In Meso) Method for Crystallizing Membrane and Soluble Proteins and Complexes htm pdf
11:10 Edward Pryor
The importance of detergent selection: Are you choosing the right detergent for your membrane protein? htm pdf


Break, refreshment

chair: Terese Bergfors

12:00 Naomi Chayen
Imperial College, London
Enhancing the success of crystallizing biological macromolecules htm pdf

12:50-14.00 Lunch buffet

S2 - Crystallization of macromolecular complexes

chair: Song Tan

 14:00 Anthony Kossiakoff 
University of Chicago, Chicago, USA
Generation of antibody-Fab reagents to capture and stabilize functionally important conformational states of proteins to facilitate their structure determination by crystallography and single particle Cryo-EM htm pdf
14:30 Nicolas Thoma 
Friedrich Miescher Institute, Basel, Switzerland
Macromolecular machines in genome maintenance htm pdf
15:00 Song Tan
Penn State University, University Park, USA
Crystallization of chromatin complexes htm pdf
15:30 Seth Rubin
University of California, CA, USA
X-ray Crystallography Studies of Protein Complexes Controlling Cell Cycle Gene Expression htm pdf
15:50 Break, refreshment      

S3 - Membrane protein crystallization II

chair: Hartmut Luecke 

16:20 Fei Xu
iHuman Institute
Shangai Tech University
New advances in structural discovery of human G protein-coupled receptors: the 826 project and importance of ligand stabilization htm pdf
16:50 Khyati Kapoor
University of California, CA, USA
Mechanism of inhibition of hGLUT1 is conserved between cytochalasin B and phenylalanine amides htm pdf
17:10 Chun Jung Chen
National Synchrotron Radiation Research Center

Hsinchu, Taiwan
Purification and Crystallization of an Antigenic Outer-Membrane Protein from Salmonella Typhi htm pdf
17:30 Patrick Shaw Stewart 
Douglas Instruments Ltd, Hungerford, UK
Microseed matrix-screening (rMMS): introduction, theory, practice and a new technique for membrane protein crystallization in LCP htm pdf

18:00 - 19:30

Poster session I


Monday, July 4

S4 - Automation in crystallization

chair: Janet Newman 

 8:30 Jose A. Marquez 
EMBL Grenoble Outstation, France
Automated crystal mounting and processing through laser photoablation. 
New opportunities for integrated macromolecular crystallography pipelines
htm pdf
9:00 Moritz Hunkeler 
University of Basel, Switzerland
Advanced Imaging in Lab-Scale Protein Crystallization htm pdf
9:30 Carien Dekker
Novartis, Basel, Switzerland
TeXRank: Texture Image Analysis and Machine Learning for Crystallizing Difficult Proteins htm pdf
10:00 Charline Gerard
CINaM - CNRS, Aix-Marseille Université
Microfluidic platform for optimisation of crystallisation conditions  htm pdf
10:20 Jochen Müller-Dieckmann
Advancements in Automated Imaging   htm   pdf


Break, refreshment

S5 - Crystallization approaches 
        for serial crystallography

chair: Christian Betzel 

 11:10 Dominik Oberthuer 
Center for Free-Electron Laser Science, Hamburg
Changing concepts: Crystallization for serial and time-resolved crystallography htm pdf
11:40 Robert Dods 
University of Gothenburg, Sweden
Micro-crystallisation of Photosynthetic Reaction Center for Time-Resolved Serial Femtosecond Crystallography at an X-ray Free Electron Laser htm pdf
12:10 Jason Stagno
National Cancer Institute, Frederick, MD, USA
Advantages of Serial Femtosecond Crystallography for RNA Structure Determination htm pdf
12:30 Sarah Perry
University of Massachusetts Amherst, USA
Graphene-Based Microfluidics for Serial Crystallography htm pdf

13:00-14.00 Lunch buffet

S6 - Crystal to beam

chair: Dominik Obertuer  

 14:00 Alke Meents 
DESY, Hamburg
A low background sample holder for fixed target serial crystallography experiments htm pdf
14:30 Uwe Weierstall
Arizona State University, AR, USA
Microcrystal sample delivery for serial crystallography in a high viscosity medium htm pdf
15:00 Christian Betzel 
University of Hamburg, Germany
Distinguishing Protein Nanocrystals from Amorphous Precipitate by Depolarized Dynamic Light Scattering htm pdf
15:30 Allen Orville
Diamond Light Source, Didcot, UK
Acoustic droplet ejection: from crystallization through time-resolved SFX htm pdf
16:00 Vernon Smith
The changing role of in-house crystallography htm pdf
16:20 Coffee, refreshment      


chair:  Naomi Chayen 

18:45 Ada Yonath
Weizmann Institute of Science, Rehovot, Israel
The Recent Resolution Revolution & Friendly Medicine htm pdf

Ars Instrumentalis Pragensis

cinema hall of hotel Pyramida PDF

Tuesday, July 5

S7 - Chemistry of crystallization

chair: Naomi Chayen 

 8:30 Terese Bergfors 
Uppsala University, Sweden
Strategies and stories for the small-scale crystallization laboratory htm pdf
 9:00 Emmanuel Saridakis 
Institute of Nanoscience and Nanotechnology, National Centre for Scientific Research ‘Demokritos’ in Athens, Greece
Thermodynamic stabilization, entropy, and crystallization of proteins htm




 9:30 Lisza M. Bruder
University of Lethbridge, Lethbridge, Canada
Glycerol alters substrate binding in PTPLP:IP complexes htm


 9:50 Jindřich Hašek
Institute of Biotechnology, Czech Academy of Sciences, Vestec
Controlling the crystal growth. Why is poly(ethyleneglycol) the most effective precipitant for protein crystallization? htm


10:10 Alexander McPherson
University of California, Irvine, CA, USA
Experiments on the Diffusion of Dyes and Ions into Protein Crystals htm



Break, refreshment

S8 - Complementary methods

chair: Claude Sauter 

 11:00 Eric Ennifar
Architecture et Réactivité de l’ARN, Université de Strasbourg, IBMC, CNRS, Strasbourg, France
ITC-assisted crystallization of protein-ligand complexes


htm pdf
11:30 Hartmut Luecke
University of California, Irvine, CA, USA
Membrane Protein Crystallization Using Cubic Lipid Phases, Bicelles and Vapor Diffusion htm




12:00 Abel Moreno
Universidad Nacional Autonoma de Mexico (UNAM), Mexico City, Mexico
Myths and realities about the influence of electric and magnetic fields on protein crystallization and protein crystal growth htm




12:20 Marko Ristic
CSIRO Manufacturing Biomedical, Melbourne, Australia
Computational tools to aid crystallization htm pdf
12:40 Thomas Jocks
Wyatt Technology EU
How light scattering techniques can contribute to purification, characterization and crystallization of proteins htm pdf

13:00-14.00 Lunch buffet

S9 - Scoring methods

chair: Jeroen Mesters 

14:00 Janet Newman 
SCIRO Parkville, Australia
What’s in a drop? Moving from images to outcomes htm pdf
14:30 Julie Wilson 
University of York, UK
Automated scoring of crystallization experiments using multiple images htm pdf
15:00 Samyam Acharya
University of Alabama, Huntsville, AL, USA
A genetic algorithm for the optimization of protein crystallization screening htm pdf
15:20 Angela Criswell
Rigaku Oxford Diffraction
An add-on device for automated in situ diffraction screening htm pdf


Break, refreshment

S10 - Teaching macromolecular crystallization

chair: Juan Manuel Garcia-Ruiz 

 16:10 Claude Sauter
University of Strasbourg, ARN - IBMC - CNRS, Strasbourg, France
Biological crystallization: from the classroom to the bench htm pdf
16:40 Martin Caffrey
Trinity College, Dublin, Ireland
A Laboratory Course on Crystallizing Membrane and Soluble Proteins and Complexes by the Lipid Cubic Phase (In Meso) Method htm pdf
17:10 Stephane Veesler
CINaM - CNRS, Aix-Marseille Université, Marseille, France
Teaching (macromolecular) crystallization with movies  htm pdf
17:30 Monika Spano
Institut de Biologie Structurale (Université Grenoble Alpes), France
Optimization of Crystallization using Dialysis Combined with Temperature Control htm pdf
17:50 Juan Manuel Garcia-Ruiz
CSIC-University of Granada, Granada. Spain
Teaching protein crystallization at the Laboratory for Crystallographic Studies (Granada, Spain) htm pdf


Poster session II



IOCBr meeting


Wednesday, July 6


chair: Jose A. Gavira



Juan Manuel Garcia-Ruiz
Laboratorio de Estudios Cristalográficos 
IACT, (CSIC-UGR), Granada, Spain
The impact of crystals in art and mind htm pdf

S11 - Crystallization methods for neutron diffraction

chair: Monika Budayová-Spano

9:20 Shigeru Sugiyama
Osaka University, Japan
Development of protein seed crystals reinforced with high-strength hydrogels htm pdf
9:50 Matthew P. Blakeley 
Institut Laue Langevin, Grenoble, France
Neutron cryo-crystallography: methods, applications and challenges htm pdf
10:20 Flora Meilleur 
North Carolina State University, Neutron Sciences, Oak Ridge National Laboratory
Crystallization of a glycosylated protein, protein expressed with Pichia pastoris, for neutron crystallography    
10:50 Niels Junius
Institut de Biologie Structurale, CEA-CNRS-UGA,Universite Grenoble Alpes, France
Crystallization with an automated apparatus for temperature-controlled flow-cell dialysis with real-time visualization  htm pdf


Break, refreshment

chair: Juan Manuel Garcia Ruiz

11:40 Richard Giège 
IBMC du CNRS, Strasbourg, France
What biocrystallogenesis tells us – What is needed in the future htm pdf

12:30-14.00 Lunch buffet



Special conference train to Pilsen

departure from Prague main railway station 15:12    


Conference dinner and 
excursions to the brewery

Pilsen brewery    
22:15   return from Pilsen 21:09    

Thursday, July 7

S12 - Theory and practice of crystallization

chair: Jindřich Hašek 

8:30 Peter Vekilov
University of Houston, TX, USA
Are the protein pre-nucleation clusters equilibrium structures or irreversible aggregates? 


8:50 David Hargreaves
Astra Zeneca, Cambridge, UK
Antibodies as Chaperones in Crystallization: Parameters for Success htm pdf
9:10 Jose Antonio Gavira
Laboratorio de Estudios Cristalograficos, IACT (CSIC-UGR), Granada, Spain
Protein crystallization in hydrogels, current status and future prospect


9:30 Paul Thaw
TTP Labtech
Flying through optimization screening with dragonfly htm pdf
9:50 Ľubica Urbáníková
Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia
Protein crystallization - tricks and practice htm pdf
10:10 Guillermo Calero
University of Pittsburgh, PA, USA
Transmission electron microscopy for the evaluation and optimization of crystal growth htm pdf


Break, refreshment

S13 - Crystallization in industry and biomedicine

chair: Bernhard Rupp 

 11:00 Allan D’Arcy
Actelion Pharmaceuticals, Switzerland
A rational approach to crystallizing proteins in the pharmaceutical industry, the impact of micro seed matrix seeding htm pdf
11:30 Linda Oster Landgren
Astra Zeneca, Gothenburg, Sweden
Successful generation of structural information for fragment-based drug discovery  htm pdf
11:50 Thomas Peignier 
Space Applications Services, Zaventem, Belgium
XRayLab: an X-ray diffraction facility for the International Space Station dedicated to the study of space-grown crystals  htm pdf
12:10 Hai Hou 
Northwestern Polytechnical University, Xi'an, China
A comparative study on the diffraction quality of protein crystals obtained using the cross-diffusion microbatch and sitting-drop vapor diffusion methods htm pdf

12:30-13.30 Lunch buffet

S14 - Ionic liquids in protein crystallization

chair: Babak Minofar 

 13:30 Magdalena Kowacz 
Polish Academy of Sciences, Crakow, Poland
Ionic liquids (ILs)-water interplay in protein crystallization. From IL additives to nucleants to… htm pdf
14:00 Marc Pusey
iXpressGenes Inc., Huntersville, USA
Ionic liquids as protein crystallization additives htm pdf
14:30 Dominique Maes
Vrije Universiteit Brussel, Belgium
How camels can aid crystallization? The challenges of the de novo designed protein Octarellin. htm pd
14:50 Patrick Charbonneau
Duke University, Durham, NC, USA
Water in Protein Crystals htm pdf
15:10 Edward Petri
Faculty of Sciences, University of Novi Sad, Serbia
Use of X-ray crystallographic data for computational modeling of receptor-ligand interactions: design of steroidal inhibitors of breast and prostate cancer cell growth  htm pdf
 15:30 Announcement of poster prizes, closing      

Comments to colors

  Keynote lectures
  Speakers invited for sessions
  Contributed talks (selected from abstracts)
  Company talks