Main Topics 

Theory and practice of crystallization
  • Chemists view of the crystallization process
  • Physical methods in protein crystallization
  • Computational prediction of crystallization
  • Scoring methods for evaluation of screens efficacy 

Sample preparation and handling in advanced techniques

  • classical crystallography
  • small angle scattering (contrast in SAX)
  • time-resolved crystallography 
    (biochemical processes on ms scale)
  • femtosecond crystallography (XFEL)
  • solid state NMR
  • high resolution electron microscopy (HREM)
  • electron diffraction
  • neutron diffraction 

Crystals for “high resolution studies”

  • Diffraction quality of protein-ligand crystals (co-crystallization, soaking technigues leading to reliable identification of drug interactions)
  • Room temperature measurements and controlled 
    hydration of crystals
  • Cryo-protection, cryo-cooling and measurement 
Membrane proteins
  • Crystallization of largely hydrophobic bio-macromolecules
  • Crystallization techniques for cell receptor complexes - protein-protein molecular recognition studies

Structure diversity of bio-macromolecules

  • Polymorphy of proteins in solid state
  • Temperature induced phase transitions
  • Role of glycosylation on protein crystallization and function 

Crystallization of conformationaly unstable proteins and other crystallization resisting (challenging) bio-macromolecules

  • Targeted fragmentation, mutation, and purification
  • Post-translational modifications, fusion constructs and other special approaches
  • Co-crystallization with molecular chaperons
  • Special protein expression systems for preparation of crystallizable protein complexes

Automation techniques in crystallization

Crystallization in industry

Crystallization in drug design  

Crystallography in education